Rabbit anti-Zika Envelope (E) Glycoprotein polyclonal antibody

ZIKV virus lysate was diluted to 300 ng/well in PBS for plate coating. Anti-ZIKV E glycoprotein Pab was serially diluted semi-log from 10 µg/mL and incubated on the coated plates. The washed plate was detected with anti-rabbit IgG-HRP conjugate and TMB substrate. OD650 is reported above.

1 µg of ZIKV virus lysate (lane 1) tested under denaturing and reducing condition, was detected using the anti-ZIKV E glycoprotein Pab at 0.5 µg/mL, followed by an anti-rabbit IgG-HRP conjugate and TMB membrane substrate.

Immunogen: Peptide sequence to Zika virus (ZIKV) envelope (E) glycoprotein.

Description: Affinity-purified rabbit polyclonal antibody detects ZIKV virus lysate in Western blot and ELISA.

Supplied: 100 µg of antibody is supplied in PBS+0.02% sodium azide at a concentration of 0.820 mg/mL.

Raised in: Rabbits

Purification: Antibody is affinity purified using immobilized immunogen.

Clonality: Polyclonal

Recommended Dilutions:

ELISA: Assay-dependent dilution.

WB: Assay-dependent dilution. Internal QC demonstrates good detection of ZIKV virus lysate when using antibody at 0.5 µg/mL.

Storage: 2-3 weeks +4°C, -20°C long term

Related Products: IBT provides a wide array of anti-filovirus-specific antibodies and other infectious disease reagents. Please see our website, www.ibtbioservices.com