ZIKV virus lysate was diluted to 300 ng/well in PBS for plate coating. Anti-ZIKV E glycoprotein Pab was serially diluted semi-log from 10 µg/mL and incubated on the coated plates. The washed plate was detected with anti-rabbit IgG-HRP conjugate and TMB substrate. OD650 is reported above.
1 µg of ZIKV virus lysate (lane 1) tested under denaturing and reducing condition, was detected using the anti-ZIKV E glycoprotein Pab at 0.5 µg/mL, followed by an anti-rabbit IgG-HRP conjugate and TMB membrane substrate.
Immunogen: Peptide sequence to Zika virus (ZIKV) envelope (E) glycoprotein.
Description: Affinity-purified rabbit polyclonal antibody detects ZIKV virus lysate in Western blot and ELISA.
Supplied: 100 µg of antibody is supplied in PBS+0.02% sodium azide at a concentration of 0.820 mg/mL.
Raised in: Rabbits
Purification: Antibody is affinity purified using immobilized immunogen.
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution. Internal QC demonstrates good detection of ZIKV virus lysate when using antibody at 0.5 µg/mL.
Storage: 2-3 weeks +4°C, -20°C long term
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