FD Rapid GolgiStain Kit

Golgi-Cox impregnation1, 2 has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons and the glia.

Using the Golgi technique, subtle morphological alterations have been discovered in neuronal dendrites and dendritic spines in the brain of animals treated with drugs, as well as in the post-mortem brain of patients with neurological diseases.3, 4 However, the lack of reliability and the slow process of Golgi staining have been major obstacles to the widespread application of this technique.

The FD Rapid GolgiStain ™ kit is designed according to the principle of the methods described by Ramón-Moliner2, Glaser, and Van der Loos5.

Not only has this kit drastically improved and simplified the Golgi-Cox technique, but it has also proven to be extremely reliable and sensitive in demonstrating the morphological details of neurons and glia, especially dendritic spines. The FD Rapid GolgiStain ™ Kit has been extensively tested and used extensively in the brains of various species of animals, as well as post mortem human brain samples.

Materials required but not included:

• Bidistilled or deionized water.
• Plastic or glass tubes or vials.
• Histological equipment and supplies, including gelatin-coated microscope slides, coverslips, staining bottles, ethanol, xylene or xylene substitutes, resinous mounting medium (eg, Permount®), and a light microscope.

References:

1. Corsi P. (1987) The morphological approach to neuroanatomy of Camillo Golgi. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in CNS pathology, pp. 1-7. Berlin: Springer.
2. Ramón-Moliner E. (1970) The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer.
3. Graveland GA, Williams RS and DiFiglia M. (1985) Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington’s disease. Sciences. 227: 770-3.
4. Robinson TE and Kolb B. (1997) Persistent structural modification in the nucleus accumbens and neurons of the prefrontal cortex produced by previous experience with amphetamines. J. Neurosci. 17: 8491-7.
5. Glaser ME and Van der Loos H. (1981) Analysis of thick brain sections by computerized front-back microscopy: application of a new high clarity Golgi-Nissl stain. J. Neurosci. Meth. 4: 117-25.